99久久久无码国产精品6,朝鲜少妇xxxx做受,欧美三级在线播放,国产女人乱人伦精品一区二区

您好,歡迎進(jìn)入研域(上海)化學(xué)試劑有限公司網(wǎng)站!
一鍵分享網(wǎng)站到:
  • 資料下載DOWN

    您當(dāng)前的位置:首頁(yè) > 資料下載 > 人活化凝血因子Ⅻ(FⅫa)英文說(shuō)明書(shū)

    人活化凝血因子Ⅻ(FⅫa)英文說(shuō)明書(shū)

    發(fā)布時(shí)間: 2017/7/26  點(diǎn)擊次數(shù): 828次
    提 供 商: 研域(上海)化學(xué)試劑有限公司 資料大?。?/td>
    圖片類型: 下載次數(shù): 98
    資料類型: DOC 瀏覽次數(shù): 828
    相關(guān)產(chǎn)品:
    詳細(xì)介紹: 文件下載    

                   Human Activated coagulation factor Ⅻ (FⅫa)FOR
    RESEARCH USE ONLY

    Assay range:2.2U/L - 80U/L               96 determinations
    Purpose
    This kit allows for the determination of FⅫa concentrations in Human serum, cell culture supernates and other biological fluids

    Principle of the assay
    The kit assay Human FⅫa level in the sample, use Purified Human FⅫa antibody to coat microtiter plate wells, make solid-phase antibody, then add FⅫa to wells, Combined FⅫa antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human FⅫa in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Materials provided with the kit
    1    wash  solution    20ml×1bottle    7    Stop Solution    6ml×1 bottle
    2    HRP-Conjugate reagent    6ml×1 bottle    8    Standard(160U/L)    0.5ml×1 bottle
    3    Microelisa stripplate    12well×8strips    9    Standard diluent    1.5ml×1bottle
    4    Sample diluent    6ml×1 bottle    10    Instruction    1
    5    Chromogen Solution A    6ml×1 bottle    11    Closure plate membrane    2
    6    Chromogen Solution B    6ml×1 bottle    12    Sealed bags    1
    Specimen requirements
    1.extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
    2.Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
    Assay procedure
    1.Dilute and add sample:Dilute Original density Standard as follow table:
    80U/L    5 Standard    150μl Original density Standard+150μl Standard diluent
    40U/L    4 Standard    150μl 5 Standard+150μl Standard diluent
    20U/L    3 Standard    150μl 4 Standard+150μl Standard diluent
    10U/L    2 Standard    150μl 3 Standard +150μl Standard diluent
    5U/L    1 Standard    150μl 2 Standard +150μl Standard diluent
    2. Add sample: Set blank wells separay (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
    3. Incubate:  After closing plate with Closure plate membrane , incubate for 30 min at 37℃.
    4. Configurate liquid: 30-fold(or 20-fold) wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
    5. Washing: Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
    6. Add enzyme: Add HRP-Conjugate reagent 50μl to each well, except  blank well. 
    7. Incubate: Operation with 3.
    8. Washing: Operation with 5.
    9. Color: Add Chromogen Solution A 50ul and Chromogen Solution B 50ul to each well, evade the light preservation for 10 min at 37℃
    10. Stop the reaction: Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).
    11. Assay: take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
    Steps description
    Standard, Sample diluent


    Add Standard, Sample diluent, incubate for 30 min at 37℃.


    Wash 5 time,Add HRP-Conjugate reagent, incubate for 30 min at 37℃.


    Wash 5 times,Add Chromogen Solution A and B, incubate for 10 min at 37℃.


    Add Stop Solution


    Read absorbance at 450nm within 15 min


    calculate
    Calculate
    Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
    Important notes
    1.The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
    2.washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
    3.add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 min, if the number of sample is much , recommend to use Volley .
    4.if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.(×n×5).
    5.Closure plate membrane only limits the disposable use, to avoid cross-contamination.
    6.The substrate evade the light preservation.
    7.Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
    8.All samples, washing buffer and each kind of reject should according to infective material process.
    9.Do not mix reagents with those from other lots.

    Storage and validity
    1.Storage:  2-8℃.
    2.validity: six months

產(chǎn)品中心 Products
在線客服 聯(lián)系方式

服務(wù)熱線

021-54479081
021-54461587

免费无码中文字幕A级毛片HD | 最近中文字幕在线资源| 亚洲av无码国产一区二区| 女人18毛片a级毛片免费视频| 野花日本大全免费观看版动漫| 精品久久久久成人码免费动漫| 久久天天躁狠狠躁夜夜av| 亚洲人成电影网站免av费| 亚洲最大成人网站| 成人免费无码成人影院日韩| 亚洲av无码一区二区乱子伦| 国产精品久久国产精品99| 国产成人盗摄精品a片在线观看| 初爱视频教程| 国产成人亚洲精品无码A大片| 天堂8中文在线最新版官网| 欧美人妻www无码国产黄漫| 色欲AV人妻精品一区二区直播| 天堂√最新版中文在线| 亚洲另类欧美综合久久图片区| 天天想你在线播放免费观看| 性按摩XXXX在线观看| 久久国产精品-国产精品| 97久久久久人妻精品专区| 一本一道VS无码中文字幕 | 中文字幕人妻丝袜乱一区三区| 欧洲美女黑人粗性暴交视频| 欧美性巨大╳╳╳╳╳高跟鞋| 婷婷精品国产亚洲av在线观看| 亚洲AV色香蕉一区二区| 亚洲欧洲日产国码无码久久99| 久久久久久亚洲精品中文字幕 | 柚子视频在线观看播放| 久久精品免费一区二区喷潮| 国内精品伊人久久久久AV| 精品国产v无码大片在线观看 | 国产乱人伦精品一区二区| 亚洲精品无码AV人在线观看国产| 亚洲精品无码午夜福利中文字幕| 无码人妻精品一区二区三区蜜桃| 破了亲妺妺的处免费视频国产|